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Disclaimer: This site does not store any files on its server. All contents are provided by non-affiliated third parties.Statistical Model Fitting of the Results of Gas Chromatography-High-Performance Liquid Chromatography-Mass Spectrometry Data.
Herein, we propose a methodology for the fitting of gas chromatography-high-performance liquid chromatography-mass spectrometry (GC-HPLC-MS) data by using non-linear regression methods, which are among the most commonly used. We consider the performance of three statistical approaches to fit a wide range of data sets that constitute different portions of the whole process, which are method optimization, key method tuning, and peak matching, respectively. The results show that all three methods worked as expected. Surprisingly, the nonlinear regression method based on local approximation was not very accurate, and was not recommended. However, it performed much better than the others in optimizing the results. We also demonstrated the importance
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In this study we have compared the dynamic distribution of intra-carotid microbubble contrast agents with that of intravenously injected, ultrasound contrast agents. Real-time images of both types of contrast agents were obtained in five open-chest, anesthetized dogs. Time-averaged concentration-time curves of the contrast agents were determined in the left common carotid artery and in several other peripheral vessels. The contrast agents caused a distribution of ultrasound backscatter in the region of the carotid bifurcation that peaked in the middle part of the common carotid artery and then decreased in the vessel walls as the contrast agents reached the level of the intervertebral space. The effect of the contrast agents on ultrasound backscatter was transient and vanished in about 5 s. In the muscle of the neck, a rapid decrease in scattering intensity was seen, probably due to washout of the contrast agents from the extracellular fluid. In the carotid body, a gradual decline in the backscatter was noted, indicating washout of the contrast agents from the bloodstream. Comparing the distribution of microbubble contrast agents with that of the ultrasound agents provides a new quantitative way to study the transport of blood in the arterial system and can be used to monitor the perfusion of tissues in vivo.
Although the majority of the patients with amyloid Aβ^+^-ASM^+^ NAWM had U-fibres, there were 2 patients with T-fibres, which may reflect the possible cross-reactivity of anti-amyloid Aβ with anti-SMA or anti-nNOS. Although there are rare reports of amyloid Aβ