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When I was a kid, my parents constantly reminded me of one thing: they were not rich. So I played my little heart out in every youth event, all along trying to prove I could give some money, even if it meant sacrificing my own fun.
Over time, this spirit of dedication to giving every dollar I could to charity became a part of my personality. Today, I still try to practice that at work and at home, donating as much as I can to charities of my choice.
When I found myself in Bangalore for a few days, I decided to share my passion with the youngsters in Bangalore. What better place than Youth League to do it?
So I went to their office and we started. The first thing on my list was to see if I could collect at least a hundred rupees from each kid there, and the donation envelope was just the right place to start.
We started by asking all the kids what they would like to do with the money. And it was great. They came up with the following ideas:
2. AIDS Healthcare Foundation [Facebook]
3. I Donate [Facebook]
4…. I Donate [Twitter]
5. The Milan’s Foundation [Facebook]
6. On 25th of November, I will collect the funds donated via
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The good quality of data from this technique suggests that this method can be used in different species and, even more important in populations that have smaller sizes as in the case of the Brazilian *M. americana* populations. We also validated the genotyping process by including a control sample (DNA from *M. americana*) with known genotypes. This control sample showed a similar performance to the samples from the 90 analyzed trees. Moreover, we attempted to eliminate the problems of null alleles and genotyping errors that can be found in microsatellite data. The PCR products for the STRs were pooled and submitted to fragment analysis, using capillary electrophoresis, where the null alleles will be removed. However, the genotyping errors, also known as stutter alleles, are a common problem that can also occur in microsatellite data and are expected to be eliminated by the software used for